2. Muscle Biopsy Kits
Muscle/Nerve biopsy kits include the following components:
Upon receipt, place the coolant pack in the freezer (no colder than -20° C) and store the remainder of the kit at room temperature (22-25° C).
3. Sample Collection
Adults: Excise a 1 cm x 1 cm x 2.5-3.0 cm longitudinal length of muscle. Do not stretch the muscle and handle gently only by the ends.
Young Children: Similarly excise and handle a 0.5 cm x 0.5 cm x 2.0-2.5 cm length of muscle.
4. Sample Preparation
Divide the biopsy, place into vials as follows, and cap all vials tightly. The plastic splint should be discarded.
A) Segment 1 (for enzyme histochemistry and immunohistochemistry)
1 cm cube (adult) or 0.5 cm cube (young children):
Remove the single 3” × 3″ gauze pad from its pouch in the sterile accessory kit and slightly dampen it with normal saline (not provided). Manually squeeze out excess saline until the gauze is just moist (not wet). Unfold the gauze to the original size, place the muscle segment near one edge and roll the gauze around the muscle to wrap it. Make a shallow fold at both ends to completely enclose the tissue. Place into the empty vial 1 (green label).
B) Segment 2 (for routine histology)
1 cm cube (adult) or 0.5 cm cube (young children):
Place this segment into vial 2 (white cap) containing 10% neutral buffered formalin.
C) Segment 3 (for EM)
No larger than 1.0 cm x 0.2 cm x 0.2 cm:
Place this segment into vial 3 (yellow cap) containing 2.5% glutaraldehyde/2.0% formaldehyde, making sure that the muscle is immersed in the fixative.
After addition of biopsy specimens, the entire box containing all vials should be prepared for shipment and kept cool with the provided coolant pack.
5. Preparation for Shipment
2. Muscle Biopsy Kits
Muscle/Nerve biopsy kits include the following components:
Upon receipt, place the coolant pack in the freezer (no colder than -20° C) and store the remainder of the kit at room temperature (22-25° C).
3. Sample Collection
Adults: Excise a 1 cm x 1 cm x 2.5-3.0 cm longitudinal length of muscle. Do not stretch the muscle and handle gently only by the ends.
Young Children: Similarly excise and handle a 0.5 cm x 0.5 cm x 2.0-2.5 cm length of muscle.
4. Sample Preparation
Divide the biopsy, place into vials as follows, and cap all vials tightly. The plastic splint should be discarded.
A) Segment 1 (for enzyme histochemistry and immunohistochemistry)
1 cm cube (adult) or 0.5 cm cube (young children):
Remove the single 3” × 3″ gauze pad from its pouch in the sterile accessory kit and slightly dampen it with normal saline (not provided). Manually squeeze out excess saline until the gauze is just moist (not wet). Unfold the gauze to the original size, place the muscle segment near one edge and roll the gauze around the muscle to wrap it. Make a shallow fold at both ends to completely enclose the tissue. Place into the empty vial 1 (green label).
B) Segment 2 (for routine histology)
1 cm cube (adult) or 0.5 cm cube (young children):
Place this segment into vial 2 (white cap) containing 10% neutral buffered formalin.
C) Segment 3 (for EM)
No larger than 1.0 cm x 0.2 cm x 0.2 cm:
Place this segment into vial 3 (yellow cap) containing 2.5% glutaraldehyde/2.0% formaldehyde, making sure that the muscle is immersed in the fixative.
After addition of biopsy specimens, the entire box containing all vials should be prepared for shipment and kept cool with the provided coolant pack.
